E0287

Carboxylesterase 1 isoform b human

recombinant, expressed in baculovirus infected BTI insect cells

• Sinónimos: Carboxylesterase 1 human, carboxylesterase, esterase
• UNSPSC Code: 12352204
• EC Number: 232-773-7
• NACRES: NA.54
• Número CE: 3.1.1.1 (BRENDA, IUBMB)
• Specific activity: ≥500 units/mg protein
• Recombinant: expressed in baculovirus infected BTI insect cells
• Concentration: 5 mg/mL

Propiedades

• recombinant: expressed in baculovirus infected BTI insect cells
• Quality Segment: 200
• form: liquid
• specific activity: ≥500 units/mg protein
• concentration: 5 mg/mL
• weight: (0.5 ml)
• shipped in: dry ice
• storage temp.: −70°C

Descripción

General description

Carboxylesterase 1 is a member of a large multigene carboxylesterase family. These enzymes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolyze long-chain fatty acid esters and thioesters. This enzyme is known to hydrolyze aromatic and aliphatic esters and is necessary for cellular cholesterol esterification. It may also play a role in detoxification in the lung and/or protection of the central nervous system from ester or amide compounds.

Carboxylesterase (CE) belongs to serine hydrolase family. It is widely present in the human body. Five isoforms of carboxylesterase is present in humans. It is encoded by CES1 gene mapped to 16q22.2 in human chromosome.

Application

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

Carboxylesterase 1 isoform b human has been used in the reaction to convert Amplex red to resorufin in horse radish peroxidase and oxygen independent manner. It has also been used in the development of an emission ratiometric two-photon probe (SE1) for quantitation in situ.

Biochem/physiol Actions

Carboxylesterase (CE) is implicated in drug metabolism in liver and hepatic steatosis. Deficiency of CE in humans causes obesity, hyperlipidemia, insulin insensitivity, and cancer.

Other Notes

One unti will hydrolyze one nanomole of 4-nitrophenyl acetate per minute at pH 7.4 at 37°C

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable