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D5758

Sigma-Aldrich

Dietilpirocarbonato

Diethyl pyrocarbonate
1 of 2 reviewers received a sample product or took part in a promotion

96% (NT)

Sinónimos:

DEP, DEPC, Dietildicarbonato, Dietiloxidiformato, Ácido etoxifórmico anhidro

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5 ML
$65.600
25 ML
$232.000
50 ML
$281.000
100 ML
$515.000

$65.600

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5 ML
$65.600
25 ML
$232.000
50 ML
$281.000
100 ML
$515.000

About This Item

Fórmula lineal:
O(COOC2H5)2
Número de CAS:
1609-47-8
Peso molecular:
162.14
Beilstein:
637031
Número CE:
216-542-8
Número MDL:
MFCD00009106
Código UNSPSC:
12352202
ID de la sustancia en PubChem:
24893964
NACRES:
NA.77

$65.600

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origen biológico

synthetic (microbial fermentation/ cell culture)

Ensayo

96% (NT)

Formulario

liquid

índice de refracción

n20/D 1.398 (lit.)

bp

93-94 °C/18 mmHg (lit.)

densidad

1.101 g/mL at 25 °C (lit.)

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

cadena SMILES

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

Clave InChI

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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Descripción general

El dietilpirocarbonato (DEPC) es un compuesto químico que se utiliza para inactivar las enzimas RNasas y es sensible a la humedad y el pH. Se descompone a 155°C a etanol y dióxido de carbono en disolución acuosa. El DEPC es también sensible al amoniaco, que provoca su descomposición a uretano, un posible carcinógeno.

Aplicación

El dietilpirocarbonato se ha utilizado en la reacción de PCR para tratar el agua desionizada, lo que reduce el riesgo de que el ARN sea degradado por las RNasas.[1] También se utiliza para la hibridación por puntos, para diluir el ARN aislado de los diferentes microorganismos.[2]
Reactivo de modificación para residuos de His y Tyr en proteínas. Sonda robusta para alteraciones estructurales en el ADN bicatenario, que reacciona con las bases total o parcialmente desapiladas.

Acciones bioquímicas o fisiológicas

El DEPC es un potente inhibidor de nucleasas.[3][4]Se utiliza una disolución al 0,1% de DEPC para inactivar la RNasa.
Inhibe la rianodina uniéndose a los receptores de rianodina/canales de Ca2+ en el músculo esquelético de una manera dependiente de la dosis y del tiempo, y aumenta la permeabilidad al Ca2+ de las vesículas SR.[5]
Inactiva la ARNasa en disolución aproximadamente al 0,1% (v/v). Por tanto, protege el ARN contra la degradación.

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H302

Clasificaciones de peligro

Acute Tox. 4 Oral

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

156.2 °F - closed cup

Punto de inflamabilidad (°C)

69 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
WXBF4721V
WXBF3527V
WXBF3675V
BCCF7889
BCCJ8445

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H Perry-O'Keefe et al.
Journal of applied microbiology, 90(2), 180-189 (2001-02-13)
A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of
Properties of mouse globin messenger ribonucleic acid and its preparation in milligram quantities.
R Williamson et al.
Biochemistry, 10(16), 3014-3021 (1971-08-03)
V Shoshan-Barmatz et al.
The Biochemical journal, 299 ( Pt 1), 177-181 (1994-04-01)
Exposure of junctional sarcoplasmic reticulum (SR) membranes or purified ryanodine receptor to the histidine-specific reagent diethyl pyrocarbonate (DEPC) led to concentration- and time-dependent inactivation of ryanodine binding. The pH-dependence of the inactivation of ryanodine binding by DEPC and the reversal
E C Williamson et al.
Journal of clinical microbiology, 39(1), 47-50 (2001-01-04)
Respiratory tract colonization with Scedosporium apiospermum in patients with chronic suppurative lung disease is a significant concern for lung transplantation candidates, since Scedosporium infections occurring posttransplantation are usually untreatable. Up to 10% of patients with cystic fibrosis attending our respiratory
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Injury and inflammation cause tissue acidosis, which is a common feature of various painful conditions. Acid-Sensing Ion channels (ASICs) are amongst the main excitatory channels activated by extracellular protons and expressed in the nervous system. Six transcripts of ASIC subunits
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Artículos

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

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Questions

1–9 of 9 Questions  

  1. What is the solubility and solution stability of Diethyl pyrocarbonate, DEPC?

    1 answer

    1. DEPC can be solubilized in 95% ethanol, e.g. 1.6 mL in 4 mL ethanol gives a clear, colorless solution. DEPC is soluble in alcohols, esters, ketones and other hydrocarbons, but has limited solubility in water (~0.1%). 6 DEPC hydrolyzes in aqueous solution, to form ethanol and carbon dioxide (CO2). Typically a 0.1% DEPC solution (1 mL of DEPC diluted in water to 1 L) is used to inactivate RNase. The DEPC will not immediately dissolve, as evidenced by the appearance of globules. The mixture should be stirred until the globules disappear. DEPC is sensitive to pH. In phosphate buffer at 25 °C, its half-life is 4 minutes at pH 6, and 9 minutes at pH 7. Hydrolysis is accelerated by Tris buffer,7 where at 25 °C, the half-life of DEPC was reported to be 1.25 minutes at pH 7.5, and 0.37 minutes at pH 8.2. DEPC will decompose in solution when autoclaved. For 0.1% DEPC solutions, autoclaving for 15 minutes per liter should be sufficient.
      DEPC is very sensitive to moisture. As such, DEPC is packaged under argon to help reduce exposure to moisture. If DEPC is exposed to even traces of moisture, some hydrolysis occurs. The resulting CO2 is more soluble in DEPC solutions at 2–8 °C than at room temperature. As the product is brought to room temperature before opening, the DEPC can become supersaturated with respect to any dissolved gas. After opening the bottle for the first time and each time thereafter, layer nitrogen or argon gas over the DEPC and store the closed bottle at 2–8 °C for optimal stability. It may be helpful to store the bottle inside a sealed plastic bag with desiccant, but with the bottle cap slightly loose. If the bag inflates at all, this indicates some degree of decomposition with possible pressure build-up. Once opened, the bottle should not be kept for more than 9 months. DEPC decomposes at 155 °C. DEPC is also sensitive to ammonia, which causes decomposition to urethane.

      Helpful?

  2. Is 121 °C - 132 °C of autoclave temperature for more than 15 min enough to degrade DEPC?

    1 answer

    1. DEPC will decompose in solution when autoclaved. For 0.1% DEPC solutions, autoclaving for 15 minutes at per liter at 121 °C.

      Helpful?

  3. How can I tell if Product D5758, Diethyl pyrocarbonate, has degraded?

    1 answer

    1. DEPC breaks down to ethanol and carbon dioxide.  The carbon dioxide builds up pressure inside the bottle; when opened, it will degas and/or bubble out of the bottle.

      Helpful?

  4. What type of container should I use to handle Product D5758, Diethyl pyrocarbonate (DEPC)?

    1 answer

    1. Glassware is preferable since some plastics, e.g.., polycarbonate, are known to react with DEPC.

      Helpful?

  5. What is the best way to use Product D5758, Diethyl pyrocarbonate, to inactivate RNAse?

    1 answer

    1. Add DEPC directly to the water to yield a 0.1 % solution.  Shake vigorously and allow to set overnight at room temperature with the lid loose or incubated for 2 hours at 37 °C.  The solution is then autoclaved 15 minutes for each liter.

      Helpful?

  6. What is the stability of Product D5758, Diethyl pyrocarbonate, at room temperature?

    1 answer

    1. The material is stable at room temperature as long as it is protected from moisture.  We do recommend 2-8 °C for its storage.

      Helpful?

  7. What buffers can I use with Product D5758, Diethyl pyrocarbonate (DEPC)?

    1 answer

    1. DEPC reacts with amines, thiols, and alcohols.  Any buffer that contains an amine, e.g.., Trizma, should not be used.  View this information on the product information sheet (under Documents, above.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Why is stirring or shaking necessary when making RNAse-free water for use with Product D5758, Diethyl pyrocarbonate?

    1 answer

    1. DEPC is not very soluble in water. Stirring and/or shaking is necessary to suspend DEPC in water.

      Helpful?

Reviews

1 of 2 reviewers received a sample product or took part in a promotion

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  1. Israel
    • Review 1
    • Votes 0
    5 out of 5 stars.

    A Decade of Excellence: A Technical Review of Sigma Products for Research

    My decade-long experience with Sigma products has been nothing short of exceptional. The consistently high quality, diverse product range, prompt delivery, and exemplary customer care support have made Sigma an indispensable ally in my research journey. I wholeheartedly recommend Sigma products to fellow researchers seeking reliable and high-quality materials for their scientific endeavors. As I look forward to the future of my research, Sigma will undoubtedly continue to be a cornerstone of my laboratory supplies.

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  2. 1 Ratings-Only Review

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