K1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92030501, human thyroid, Not specified

• UNSPSC Code: 41106514
• Biological source: human thyroid
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Not specified
• Manufacturer/tradename: ECACC

Propiedades

• biological source: human thyroid
• description: Human thyroid carcinoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 92030501-DNA-5UG, pkg of vial of cells 92030501-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Wild-type p53 tumour suppressor gene
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Application

K1 Cell Line human has also been used to study and correlate the expression levels of c-KIT (a receptor tyrosine kinase) and two markers of thyrocyte differentiation (PAX8 and TTF-1).

Tumourigenicity studies, particularly the role of p53

Biochem/physiol Actions

Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene. It has been reported that K1 cells have their origin in the thyroid papillary carcinoma cell line GLAG-66.

Human thyroid carcinoma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11,12
D5S818: 10,11
D7S820: 11
THO1: 6,9
TPOX: 8
vWA: 17,18

Preparation Note

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.