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C6295

Cell Freezing Medium-DMSO Serum free 1x

sterile-filtered, suitable for cell culture

Sinónimos:

cryopreservation medium

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A ustedes/SKUDisponibilidadPrecio
50 mL
Comprobar disponibilidad del carrito
$129.000

Acerca de este artículo

NACRES:
NA.75
UNSPSC Code:
12352207

$129.000


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sterility

sterile-filtered

Quality Segment

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

General description

Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.

Application

Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.

Other Notes

Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.

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Este artículo
C9249C6164C6039
technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200


Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.

    1 answer
    1. The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf

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