A2554
Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Goat Anti-Mouse IgG (Fc specific)–HRP
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About This Item
Productos recomendados
origen biológico
goat
Nivel de calidad
conjugado
peroxidase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
reactividad de especies
mouse
no debe reaccionar con
bovine, human, horse
técnicas
direct ELISA: 1:70,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Especificidad
Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.
Aplicación
Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in:
- immunohistochemistry
- enzyme-linked immunosorbent assay (ELISA)
- immunoblot
- dot-blot analysis
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Acciones bioquímicas o fisiológicas
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity.
Otras notas
Antibody adsorbed with bovine, equine and human serum proteins.
Forma física
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Nota de preparación
Adsorbed to reduce background staining with bovine, horse, or human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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