79390

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• Sinónimos: Alkaline Phosphatase from calf intestinal mucosa
• Número de CAS: 9001-78-9
• Comisión internacional de enzimas: 3.1.3.1 (BRENDA, IUBMB)
• Número CE: 232-631-4
• Número MDL: MFCD00131849
• Código UNSPSC: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

Propiedades

• origen biológico: bovine (calf) intestinal mucosa
• Formulario: solution (clear, colorless)
• actividad específica: ~2500 U/mg protein (~10 mg/ml)
• mol peso: M_r ~140000
• técnicas: enzyme immunoassay: suitable
• impurezas: 0.1 mM ZnCl₂; 1 mM MgCl₂; 3 M NaCl; 30 mM triethanolamine
• pH: ~7.6
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Acciones bioquímicas o fisiológicas

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Definición de unidad

1 U entspricht der Enzymmenge, die 1 μmol 4-Nitrophenyl-phosphat pro Minute bei pH 9.8 und 37°C hydrolysiert

Reconstitución

The solution can be used without dialysis (for enzyme immuno-assay).

Otras notas

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)