79390

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• Sinónimos: Alkaline Phosphatase from calf intestinal mucosa
• Número CAS: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• EC Number: 232-631-4
• NACRES: NA.54
• MDL number: MFCD00131849
• Número CE: 3.1.3.1 (BRENDA, IUBMB)
• Specific activity: ~2500 U/mg protein (~10 mg/ml)
• Biological source: bovine (calf) intestinal mucosa

Propiedades

• biological source: bovine (calf) intestinal mucosa
• form: solution (clear, colorless)
• specific activity: ~2500 U/mg protein (~10 mg/ml)
• technique(s): enzyme immunoassay: suitable
• impurities: 0.1 mM ZnCl₂, 1 mM MgCl₂, 3 M NaCl, 30 mM triethanolamine
• pH: ~7.6
• storage temp.: 2-8°C

Descripción

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Preparation Note

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)