If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
E7750
N-(3-dimetilaminopropilo)-N′-etilcarbodiimida hydrochloride
commercial grade, powder
Sinónimos:
N-etil-N′-(3-dimetilaminopropil)carbodiimida hydrochloride, EDAC, EDC, EDC hydrochloride, WSC hydrochloride
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|
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Quality Segment
form
powder
technique(s)
Northern blotting: suitable, bioconjugation: suitable
color
white to off-white
mp
110-115 °C (lit.)
solubility
H2O: ≤100 mg/mL
storage temp.
−20°C
SMILES string
Cl.CCN=C=NCCCN(C)C
InChI
1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key
FPQQSJJWHUJYPU-UHFFFAOYSA-N
General description
La versatilidad del HCl de EDC se manifiesta aún más en su capacidad de modificar los ácidos nucleicos, permitiendo el marcaje del ADN y el ARN a través de sus grupos de 5′ fosfato. Esta funcionalidad mejora la visualización, el seguimiento y el análisis de estas moléculas cruciales y contribuye de manera significativa al avance de la investigación de los ácidos nucleicos. Además, el HCl de EDC sirve como un puente biomolecular vital, actuando como un reticulante que conecta los NHS-ésteres reactivos a las aminas de las biomoléculas con los grupos carboxilo. Esta técnica es particularmente valiosa en la conjugación de proteínas, permitiendo la creación de moléculas híbridas con nuevas propiedades y funciones. El mecanismo subyacente del HCl de EDC implica su reacción con un grupo carboxilo, formando un intermediario inestable que busca activamente un compañero amina. El delicado equilibrio de esta reacción enfatiza la necesidad de optimizar las condiciones que garanticen una conjugación eficaz. La asistencia de la N-hidroxisuccinimida (NHS) potencia más las capacidades del HCl de EDC al estabilizar el intermediario y permitir procedimientos de conjugación en dos etapas, ofreciendo una mayor flexibilidad y control, en especial cuando se trata de biomoléculas complejas.
Application
- El clorhidrato de N-(3-dimetilaminopropil)- N′-etilcarbodiimida se ha utilizado para la formación de bioconjugados de FND (nanodiamantes fluorescentes)-transferrina.
- Se ha utilizado para el reticulado de la polietilenimina con las partículas de oro.[1]
- Se ha utilizado como agente de unión a la carbodiimida para el recubrimiento de microesferas de poliestireno carboxilado con BSA (seroalbúmina sérica) biotinilada.
Biochem/physiol Actions
Features and Benefits
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| form powder | form crystalline | form powder | form solid |
| technique(s) Northern blotting: suitable, bioconjugation: suitable | technique(s) - | technique(s) bioconjugation: suitable | technique(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C (−15°C to −25°C) |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility H2O: ≤100 mg/mL | solubility H2O: ≤100 mg/mL | solubility H2O: soluble 0.2 g/L | solubility - |
| color white to off-white | color white to off-white | color - | color - |
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signalword
Danger
target_organs
Stomach,large intestine,lymph node
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| E7750-10G | 04061835516018 |
| E7750-5G | 04061835538379 |
| E7750-1KG | 04061835544653 |
| E7750-5KG | 04061832862132 |
| E7750-100MG | 04061835544646 |
| E7750-1G | 04061835483433 |
| E7750-25G | 04061835544660 |
| E7750-100G | 04061835544639 |
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How can I determine the shelf life / expiration / retest date of this product?
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Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What impurities might be present in N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750?
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The two compounds most likely to appear in EDAC as trace impurities are 3-dimethylaminopropylamine and N-(3-dimethylaminopropyl)-N'-ethylurea as a result of synthesis (intermediate) and degradation (moisture or coupling reaction), respectively.
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Product No. E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, is listed as "commercial grade". What does that mean?
1 answer-
It means that it is the grade of N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide (EDAC) hydrochloride that was commercially available at the time we purchased it. This product is not assayed for purity. If you need a high purity EDAC, consider Product No. 03450.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750, be used as a peptide coupling reagent in alcoholic solvents? If so, do you have a reference?
1 answer-
Yes. Nosaki, S., Effects of amounts of additives on peptide coupling mediated by a water-soluble carbodiimide in alcohols. J. Peptide Res., 54(2), 162-167 (1999). This paper describes using the E7750 along with 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HOOBt) and 1-hydroxy-7-azabenzotriazole (HOAt) for enhancement of peptide coupling.
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I understand that E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, can be used in peptide synthesis. Do you have a brochure on some other reagents that can be used for peptide synthesis?
1 answer-
Yes.
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