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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 mL | Comprobar disponibilidad del carrito | $77.100 |
5 mL | Comprobar disponibilidad del carrito | $368.000 |
10 mL | Comprobar disponibilidad del carrito | $695.000 |
Acerca de este artículo
$77.100
shelf life
≤12 mo.
manufacturer/tradename
Roche
packaging
kit of 1 mL (100 x 20 μL rxn; KK4705), kit of 10 mL (1000 x 20 μL rxn; KK4707), kit of 5 mL (500 x 20 μL rxn; KK4706), kit of 50 mL (5000 x 20 mL rxn; KK4714), pkg of
concentration
2 ×
technique(s)
qPCR: suitable
compatibility
for use with Applied Biosystems® 5700, for use with Applied Biosystems® 7000, for use with Applied Biosystems® 7300, for use with Applied Biosystems® 7700, for use with Applied Biosystems® 7900HT, for use with StepOne™, for use with StepOnePlus™
storage temp.
−20°C
General description
Application
Features and Benefits
- Exceptional differentiation of heterozygous and homozygous alleles
- Discrete clusters in SNP genotyping assays
Achieve excellent reproducibility and efficiency:
- Fast, high performance five-colored multiplex PCR
- Similar abundance levels achieved with low copy number
- Broad dynamic range of up to 10 orders of magnitude
Increase flexibility:
- Compatible with both standard and fast cycling protocolsRetain accurate quantification
Quick Notes:
- These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
- Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
- For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
- Do not exceed 25 μL volumes.
- Compatible with different qPCR protocols
Preparation Note
KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM® is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.
Analysis Note
Other Notes
Legal Information
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) RT-qPCR: suitable |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shelf life ≤12 mo. | shelf life ≤12 mo. | shelf life ≤12 mo. | shelf life - |
| packaging kit of 1 mL (100 x 20 μL rxn; KK4705), kit of 5 mL (500 x 20 μL rxn; KK4706), pkg of , kit of 10 mL (1000 x 20 μL rxn; KK4707), kit of 50 mL (5000 x 20 mL rxn; KK4714) | packaging kit of 1 mL (100 x 20 μL rxn; KK4716), kit of 10 mL (1000 x 20 μL rxn; KK4718), kit of 5 mL (500 x 20 μL rxn; KK4717), pkg of | packaging kit of 1 mL (100 x 20 μL rxn; KK4701), kit of 10 mL (1000 x 20 μL rxn; KK4703), kit of 5 mL (500 x 20 μL rxn; KK4702), kit of 50 mL (5000 x 20 μL rxn; KK4715) | packaging pkg of 500 RXNS |
| concentration 2 × | concentration 2 × | concentration 2 × | concentration - |
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Solo componentes del kit
- qPCR Master Mix
- ROX™ High incorporated in the reaction mix
- KAPA Taq HotStart® DNA Polymerase
- dNTPs
- MgCl2 5 nM
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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