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FIBRP-RO

Roche

Fibronectina

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

Sinónimos:

Fibronectin

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About This Item

Número MDL:
MFCD00131062
Código UNSPSC:
12352202

origen biológico

human plasma

Nivel de calidad

100

esterilidad

non-sterile; 0.2 μm filtered

Ensayo

>95% (SDS-PAGE)

Formulario

lyophilized (clear, colorless solution after reconstitution)

mol peso

440 kDa

envase

pkg of 1 mg (11051407001)
pkg of 5 mg (11080938001)

fabricante / nombre comercial

Roche

técnicas

cell culture | mammalian: suitable

Nº de acceso UniProt

P02751

temp. de almacenamiento

2-8°C

Información sobre el gen

human ... FN1(2335)

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Descripción general

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Especificidad

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Aplicación

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

Secuencia

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Forma física

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Nota de preparación

Working concentration: 5μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitución

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

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Certificados de análisis (COA)

Lot/Batch Number
61285900
57298100
53266900
37816200
35338500

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Roberta Bulla et al.
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Complement C1q is the activator of the classical pathway. However, it is now recognized that C1q can exert functions unrelated to complement activation. Here we show that C1q, but not C4, is expressed in the stroma and vascular endothelium of
Anindita Dutta et al.
International journal of clinical and experimental pathology, 3(3), 288-302 (2010-03-13)
The interaction of cells with adhesion proteins in the extracellular matrix (ECM) provides signals which affect the morphology, motility, gene expression and survival of adherent cells. In the present communication we cultured K562 cells in presence of fibronectin to study
Benjamin N Rollo et al.
F1000Research, 4, 113-113 (2015-06-13)
The avian enteric nervous system (ENS) consists of a vast number of unusually small ganglia compared to other peripheral ganglia. Each ENS ganglion at mid-gestation has a core of neurons and a shell of mesenchymal precursor/glia-like enteric neural crest (ENC)
Fibronectin at a glance.
Roumen Pankov et al.
Journal of cell science, 115(Pt 20), 3861-3863 (2002-09-24)

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Protocolos

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