SCT249
BioTracker™ Spermidine Polyamine Green Fluorescent Live Cell Probe
Human
Sinónimos:
clickable polyamine probe, live cell probe, polyamine probe, polyamine transport system probe, spermidine probe
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About This Item
Código UNSPSC:
41106514
NACRES:
NB.12
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Nombre del producto
BioTracker™ Spermidine Polyamine Green Fluorescent Live Cell Probe,
origen biológico
human
Nivel de calidad
envase
vial of 500 μg
fabricante / nombre comercial
Millipore
técnicas
cell culture | stem cell: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Aplicación
The relationship between polyamine systems and cancer---as well as critical normal cell processes--- led to the creation of clickable polyamine derivatives which are innovative and novel tools for functional studies of the polyamine transport system (PTS). Spermidine and other important polyamine derivatives were conjugated to a BODIPY fluorophore through click chemistry.
MW: 599.44 g/mol
SPECTRAL PROPERTIES
λex: 494 nm
λem: 508 nm
MW: 599.44 g/mol
SPECTRAL PROPERTIES
λex: 494 nm
λem: 508 nm
Características y beneficios
The BioTracker Clickable Polyamine Spermidine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine tracking with superior cellular uptake. Probe may be dissolved in DMSO to make a 10mM stock solution. 1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.
Descripción de destino
Polyamines play a variety of critical roles in the cell. Some of these processes include gene expression, cell proliferation and differentiation, and cellular stress management.
Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal system, ovary, prostate, and pancreas.
In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.
Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal system, ovary, prostate, and pancreas.
In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.
Almacenamiento y estabilidad
Store at -20 °C, protected from light
Otras notas
This spermidine derivative click-conjugated to BODIPY is optimized for the study of the polyamine transport system, which is exploited in diverse cancers.
Información legal
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Roeland Vanhoutte et al.
Chembiochem : a European journal of chemical biology, 19(9), 907-911 (2018-02-17)
Polyamines are essential for cell growth and differentiation, but their trafficking by the polyamine transport system is not fully understood. Herein, the synthesis of several azido-derivatized polyamines for easy conjugation by click chemistry is described. Attachment of a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)
Norin Nabil Hamouda et al.
The Journal of biological chemistry, 296, 100182-100182 (2020-12-15)
Polyamines, such as putrescine, spermidine, and spermine, are physiologically important polycations, but the transporters responsible for their uptake in mammalian cells remain poorly characterized. Here, we reveal a new component of the mammalian polyamine transport system using CHO-MG cells, a
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss
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