OP03
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
liquid, clone PAb421, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
PAb421, monoclonal
Formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies
rabbit, monkey, human, mouse, rat
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
do not freeze
isotipo
IgG2a
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... TP53(7157)
Descripción general
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Inmunógeno
Epitope: within amino acids 376-378 of human p53
Mouse
partially purified mouse p53
Aplicación
Frozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Envase
Please refer to vial label for lot-specific concentration.
Advertencia
Toxicity: Standard Handling (A)
Forma física
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Nota de análisis
Negative Control
SK-OV-3 cells or normal skin
SK-OV-3 cells or normal skin
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Otras notas
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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