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IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Sinónimos:

Western blotting membrane, blotting membrane, transfer membrane

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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Nombre del producto

Membrana DE PVDF Immobilon®- P, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Segment

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

La membrana de transferencia Immobilon-P se recomienda para la mayoría de las aplicaciones de inmunoelectrotransferencia, en especial para proteínas superiores a 20 kDa. La membrana Immobilon-P tiene una excelente retención de proteínas, una gran fuerza física y una amplia compatibilidad química, lo que la hace ideal para una variedad de aplicaciones de tinción y reanálisis en métodos de inmunodetección.

Features and Benefits

Membrana de PVDF Immobilon-P:
  • No se agrieta, riza ni fractura al cortarla
  • Bajo fondo
  • Capacidades de tinción superiores
  • Puede reutilizarse varias veces

Other Notes

Código del filtro: IPVH
Mecanismos de adsorción principales: Electrostático, hidrófobo
Sustituye a: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
IPVH20200IPVH08130IPVH10100
Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

compatibility

for use with Amido black, for use with Colloidal gold, for use with India ink, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination, for use with CPTS, for use with Ponceau-S red, for use with Coomassie brilliant blue, for use with Toluidine blue

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, fluorometric, colorimetric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Questions

  1. What is the recommended blocking agent for PVDF membranes in a western blot, particularly when using Casein? Is casein suitable for PVDF membranes considering its suitability as a blocking agent?

    1 answer
    1. The recommended blocking agent for PVDF membranes in a Western blot, including when using Casein, as per ther Immobilon®P Transfer Membrane user guide is using a blocking buffer called Block-CH buffer (Cat. No. WBAVDCH01) or a blocking agent such as bovine serum albumin, casein, or nonfat dry milk in wash buffer at a concentration of 0.5-5% (w/v).

      Regarding the suitability of Casein as a blocking agent for PVDF membranes, in-house testing has been conducted with IPVH00010 PVDF membrane, and Casein has been found to be suitable. However, it is important to note that users often choose the blocking agent that works best for their specific needs. Therefore, if one prefers to use Casein as a blocking agent with PVDF membrane (IPVH00010), it can be employed as long as it does not interfere with the samples and antibodies being used.

      Helpful?

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