AB5972

Anti-Dishevelled-2 Antibody

Chemicon^®, from rabbit

• Sinónimos: DVL2, DSH Homolog 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 4

Propiedades

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin (Partially purified)
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• NCBI accession no.: NM_004422.2
• UniProt accession no.: O14641
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DVL2(1856) ; mouse ... Dvl2(13543)

Descripción

General description

80 kDa

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

Immunogen

Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.

Application

Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.

Immunohistochemistry: 1:1,000 using the avidin-biotin system.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

Physical form

Format: Purified

Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Analysis Note

Control
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma

testicular teratoma) cell lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable