616387

N^α-Tosyl-Phe Chloromethyl Ketone

Irreversible inhibitor of chymotrypsin.

• Sinónimos: N^α-Tosyl-Phe Chloromethyl Ketone, TPCK
• Fórmula empírica (notación de Hill): C₁₇H₁₈ClNO₃S
• Número CAS: 402-71-1
• Peso molecular: 351.85
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00000935
• Assay: ≥98% (TLC)
• Form: solid
• Storage condition: OK to freeze

Propiedades

• Quality Segment: 100
• description: RTECS - XT5613500
• assay: ≥98% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: ethanol: 5 mg/mL, methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [S](=O)(=O)(N[C@@H](Cc2ccccc2)C(=O)CCl)c1ccc(cc1)C
• InChI: 1S/C17H18ClNO3S/c1-13-7-9-15(10-8-13)23(21,22)19-16(17(20)12-18)11-14-5-3-2-4-6-14/h2-10,16,19H,11-12H2,1H3/t16-/m0/s1
• InChI key: MQUQNUAYKLCRME-INIZCTEOSA-N

Descripción

General description

Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharide (EC₅₀ = 20 µM). Suggested working concentration is 10-100 µM.

This product has been discontinued.


Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides (EC₅₀ = 20 M).

Biochem/physiol Actions

Cell permeable: no

EC50 = 20 µM in blocking the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides.

Primary Target
chymotrypsin activity in preparations

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 4 months at 4°C.

Other Notes

Fearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Weaver, V.M., et al. 1993. Biochem. Cell Biol.71, 488.
Murakami, T., et al. 1991. Biochim. Biophys. Acta1079, 279.
Nierodzik, M.L., et al. 1991. J. Clin. Invest.87, 229.
Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Información de seguridad

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H315,H318,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable