Documentos clave

Ver Toda la documentación

Saltar a

437996

5-Lipoxygenase, Human, Recombinant, S. fruigiperda

Name: 5-Lipoxygenase, Human, Recombinant, <i>S. fruigiperda</i>
Brand: MM

5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.

Sinónimos:

5-LO, IGFBP-7

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas

A ustedes/SKU	Disponibilidad	Precio

Acerca de este artículo

Número CE:

1.13.11.34 (BRENDA, IUBMB)

NACRES:

NA.77

UNSPSC Code:

12352202

Specific activity:

~20 U/mg

En este momento no podemos mostrarle ni los precios ni la disponibilidad

Servicio técnico

¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.

Permítanos ayudarle

Propiedades

Quality Segment

100

form

liquid

specific activity

~20 U/mg

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Descripción

General description

Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A₄. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA₂.

Physical form

In 100 mM Tris containing 5 mM EGTA, pH 8.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Brock, T.G., et al. 1997. J. Biol. Chem.272, 8276.
Pouliot, M., et al. 1996. Eur. J. Biochem.238, 250.
Zhang, Y.Y., et al. 1993. J. Biol. Chem.268, 2535.
Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA81, 689.

One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo	524733	325885	524734
Sigma-Aldrich 437996 5-Lipoxygenase, Human, Recombinant, S. fruigiperda	Sigma-Aldrich 524733 Phosphodiesterase 4D3, His•Tag^®, Human, Recombinant, S. frugiperda	Sigma-Aldrich 325885 c-MET, GST-Fusion, Human, Recombinant, S. frugiperda	Sigma-Aldrich 524734 Phosphodiesterase 3B, His•Tag^®, Human, Recombinant, S. frugiperda
specific activity ~20 U/mg	specific activity ≥0.25 U/μL	specific activity ≥30 units/mg protein	specific activity ≥0.5 U/μL
form liquid	form liquid	form liquid	form liquid
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c