344826

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.

• Sinónimos: α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli, α-L-Fucoside Fucohydrolase
• Número CAS: 9037-65-4
• Número CE: 3.2.1.51 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• MDL number: MFCD01861132
• Specific activity: ≥1.0 units/mL, ≥1.5 units/mg protein
• Recombinant: expressed in E. coli

Propiedades

• recombinant: expressed in E. coli
• Quality Segment: 100
• form: liquid
• specific activity: ≥1.0 units/mL, ≥1.5 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: Proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Note: 1 mU = 1 milliunit.

Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.

Zheng, Q., et al. 2002. J. Biol. Chem.277, 39823.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 2