331569
Ethidium Bromide Adsorber
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
Sinónimos:
Ethidium Bromide Adsorber
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form
suspension
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
gray-brown
shipped in
ambient
storage temp.
15-25°C
SMILES string
C(C)c3c(cccc3)C=C.c2(c(cccc2)C=C)C=C.c1(ccccc1)C=C
InChI
1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2
InChI key
NWUYHJFMYQTDRP-UHFFFAOYSA-N
General description
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Product does not compete with ATP.
Reversible: no
Preparation Note
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Other Notes
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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