233156

Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

• Sinónimos: Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT
• Fórmula empírica (notación de Hill): C₄H₁₀O₂S₂
• Número CAS: 3483-12-3
• Peso molecular: 154.25
• UNSPSC Code: 12161504
• NACRES: NA.25
• MDL number: MFCD00004877
• Assay: ≥99% (titration)
• Form: powder, solid

Propiedades

• grade: Molecular Biology
• Quality Segment: 100
• description: Merck USA index - 14, 3376
• assay: ≥99% (titration)
• form: powder, solid
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 15 mg/mL
• cation traces: heavy metals: ≤0.001%
• absorption: ≤0.100 at 280 nm at 100 mM (1 cm), ≤0.500 at 260 nm at 100 mM (1 cm)
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChI key: VHJLVAABSRFDPM-IMJSIDKUSA-N

Descripción

General description

Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

A protective agent for SH groups.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Información de seguridad

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H314,H412
• pcodes: P260 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
• Clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable