227036

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

• Sinónimos: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
• Número CAS: 9012-63-9
• UNSPSC Code: 12352202
• NACRES: NA.51
• MDL number: MFCD00130776
• Assay: ≥95% (SDS-PAGE)
• Form: solid
• Storage condition: do not freeze

Propiedades

• Nombre del producto: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
• Quality Segment: 100
• description: RTECS - LF3100000
• assay: ≥95% (SDS-PAGE)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• solubility: soluble (Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.)
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..

Useful for tissue culture applications.

Biochem/physiol Actions

Cell permeable: no

Primary Target
α-subunit of G-proteins

Product does not compete with ATP.

Reversible: no

Packaging

Please refer to vial label for lot-specific concentration.

yes

Physical form

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.

Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

Analysis Note

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.

single band by disc gel electrophoresis

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

Legal Information

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

Información de seguridad

• Pictogramas: GHS06
• Palabra de advertencia: Danger
• Códigos de peligro: H300 + H310,H315
• Indicaciones de precaución: P262 - P264 - P270 - P280 - P301 + P310 - P302 + P352 + P310
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2
• Clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• ¿Qué está pasando?: WGK 3