219466

CELLULYSIN^™ Cellulase, Trichoderma viride

• Sinónimos: β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
• Número CAS: 9012-54-8
• Número CE: 3.2.1.4 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00081510
• eCl@ss: 32160410
• Specific activity: ≥10,000 units/g dry wt.
• Biological source: fungus (Trichoderma viride)

Propiedades

• biological source: fungus (Trichoderma viride)
• form: solid
• specific activity: ≥10,000 units/g dry wt.
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): transformation: suitable
• color: yellow-white
• input: plant, fungi and insect sample(s)
• solubility: water: soluble
• suitability: suitable for molecular biology
• application(s): food and beverages; pharmaceutical
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Application

CELLULYSIN^™ Cellulase, Trichoderma viride has been used:

• to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
• in the enzyme mix used for in situ hybridization to plant chromosomes
• in the enzyme solution for the transformation of Phytophthora species
• to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable