07-909

Anti-phospho-Src (Tyr418) Antibody

Upstate^®, from rabbit

• Sinónimos: c-Src, p60-Src Src kinase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: chicken, human, mouse
• Application: ICC, IHC, WB
• Citations: 19

Propiedades

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: chicken, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_005417.3, NM_198291.1
• UniProt accession no.: P12931
• shipped in: dry ice
• target post-translational modification: phosphorylation (pTyr418)
• Gene Information: human ... SRC(6714)

Descripción

General description

60 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)

Application

Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.

Recognizes Src phosphorylated on Tyrosine residue 418.

Physical form

Affinity Purified rabbit polyclonal IgG in PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.

ImmunoAffinity Purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein

Routinely evaluated by immunoblot.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable