07-1217
Anti-PP1β Antibody
Upstate®, from rabbit
Sinónimos:
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, Protein phosphatase 1, catalytic subunit, beta, Protein phosphatase 1, catalytic subunit, beta isoform, Protein phosphatase 1-beta, Protein phosphatase 1-delta
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
bovine, mouse, human, rat
fabricante / nombre comercial
Upstate®
técnicas
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PPP1CB(5500)
Descripción general
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Especificidad
Recognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms.
Inmunógeno
Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327).
Aplicación
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
This Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β.
Calidad
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
Descripción de destino
~ 38kDa
Ligadura / enlace
Replaces: AB4083
Forma física
Format: Purified
Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
Almacenamiento y estabilidad
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
HeLa Cell Lysate
HeLa Cell Lysate
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ruijie Liu et al.
Journal of molecular and cellular cardiology, 87, 204-213 (2015-09-04)
There are 3 protein phosphatase 1 (PP1) catalytic isoforms (α, β and γ) encoded within the mammalian genome. These 3 gene products share ~90% amino acid homology within their catalytic domains but each has unique N- and C-termini that likely
Wen Li et al.
Journal of diabetes research, 2018, 8743874-8743874 (2018-06-30)
Ingested food is received, mixed, and ground into chyme by distinct gastric motility patterns. Diabetes impairs gastric muscle function, but the mechanisms underlying diabetes-induced gastric muscle dysfunction are unknown. Here, we compared the expression and phosphorylation of Ca2+ sensitization and
Ruijie Liu et al.
Acta biochimica et biophysica Sinica, 51(3), 323-330 (2019-02-06)
Protein phosphatase 1 isoforms α, β, and γ (PP1α, PP1β, and PP1γ) are highly homologous in the catalytic domains but have distinct subcellular localizations. In this study, we utilized both primary cell culture and knockout mice to investigate the isoform-specific
Peng Xu et al.
The Journal of experimental medicine, 218(8) (2021-06-23)
Biochemical, pathogenic, and human genetic data confirm that GSAP (γ-secretase activating protein), a selective γ-secretase modulatory protein, plays important roles in Alzheimer's disease (AD) and Down's syndrome. However, the molecular mechanism(s) underlying GSAP-dependent pathogenesis remains largely elusive. Here, through unbiased
Pragya Sharma et al.
Diabetes, 65(9), 2606-2617 (2016-06-02)
Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) and Ser(588) by Akt is essential for insulin's full effect on glucose transport. However, protein phosphorylation is determined by the balance of actions by kinases and phosphatases, and the specific phosphatase(s)
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