V3638
Vascular Endothelial Growth Factor 164 from rat
>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Synonym(s):
VEGF 164, VEGF-164, VEGF164, Vasculotropin
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About This Item
Recommended Products
biological source
rat
Quality Level
recombinant
expressed in NSO cells
Assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
1.08 ng/mL ED50
mol wt
dimer 50 kDa by SDS-PAGE
monomer 25 kDa by SDS-PAGE
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Vegfa(83785)
Application
Vascular Endothelial Growth Factor 164 (VEGF 164) from rat was used for cardiomyocytes culture and Ca2+ transients′ measurement. It was used as a medium M-199 supplement utilized in in vitro incubation for examining the capability of CTS to limit the impact of biologically relevant molecules, in particular epidermal growth factor (EGF), basic fibroblast growth factor (FGF2), and vascular endothelial growth factor A (VEGFA) in rat epididymis segment 1 and 2.
Biochem/physiol Actions
Vascular Endothelial Growth Factor 164 also referred to as VEGF 164 or Vasculotropin is a growth factor that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF 164 can enhance collagen and fibronectin expression in mesangial cells which in turn stimulates TGF-β1-induced extracellular matrix. It also plays a pivotal role in progressive glomerular fibrosis.
Mitogen for endothelial cells, enhances vascular permeability, activates, and chemoattracts monocytes and is a procoagulant.
Other Notes
164 amino acid variant of rat VEGF.
Physical form
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA containing 0.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Jose L Tomsig et al.
Biology of reproduction, 75(4), 598-604 (2006-07-21)
Previous evidence has shown that sperm maturation is the result of successive events that influence sperm cells as they move through different microenvironments from the caput to the cauda epididymis. The physiological basis for the creation and maintenance of specific
Lin Wang et al.
The Journal of biological chemistry, 279(32), 33213-33219 (2004-05-15)
Transforming growth factor-beta1 (TGF-beta1) is a potent inducer of extracellular matrix synthesis leading to progressive glomerular fibrosis. The intracellular signaling mechanisms involved in this process remain incompletely understood. The p38 mitogen-activated protein kinase (MAPK) is a major stress signal transducing
Mujun Sun et al.
Frontiers in molecular neuroscience, 15, 937350-937350 (2022-11-18)
Mild traumatic brain injury (mTBI) is a common and unmet clinical issue, with limited treatments available to improve recovery. The cerebrovascular system is vital to provide oxygen and nutrition to the brain, and a growing body of research indicates that
Wolfgang Rottbauer et al.
Genes & development, 19(13), 1624-1634 (2005-07-07)
The strength of the heart beat can accommodate in seconds to changes in blood pressure or flow. The mechanism for such homeostatic adaptation is unknown. We sought the cause of poor contractility in the heart of the embryonic zebrafish with
C J Robinson et al.
Journal of cell science, 114(Pt 5), 853-865 (2001-02-22)
Vascular endothelial growth factor (VEGF) is a secreted mitogen highly specific for cultured endothelial cells. In vivo VEGF induces microvascular permeability and plays a central role in both angiogenesis and vasculogenesis. VEGF is a promising target for therapeutic intervention in
Articles
VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.
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