RAB0182

Human bFGF ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): Basic fibroblast growth factor, FGF-2, Fibroblast growth factor 2, HBGF-2, Heparin-binding growth factor 2, bFGF
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... FGF2(2247)
• Input: sample type cell culture supernatant(s); sample type serum; sample type plasma; sample type urine
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type serum; sample type plasma; sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 50 pg/mL; standard curve range: 102.4-10000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FGF2(2247)

Description

General description

The Human bFGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bFGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human basic-FGF

Application

Human bFGF ELISA kit has been used:

• to quantify the level of residual cytokines in decellularized meningeal scaffolds (DMS)[1]
• to measure cytokine levels in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (RMS/CMS)[2]
• to analyze basic-fibroblast growth factor (bFGF) concentration in cell culture medium[3]

Biochem/physiol Actions

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3.[4] It stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal and endothelial cells.[5][6] FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair.[7] FGF2 is known to stimulate angiogenesis and is associated with cancer development.[8] High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases.[9] MicroRNA (miR)-503 targets FGF2 mRNA and inhibits migration and invasiveness of osteosarcoma (OS) cells.[10]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDS
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids