NXTRACT

NuCLEAR^™ Extraction Kit

For mammalian tissue or cultured cells

• Synonym(s): Nuclear Isolation Kit
• NACRES: NA.56
• UNSPSC Code: 41105517

Properties

• Quality Segment: 300
• usage: kit sufficient for 10 extractions (1 ml packed cell volume), kit sufficient for 100 extractions (100 μl packed cell volume)
• technique(s): protein extraction: suitable, western blot: suitable
• input: mammalian sample(s)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Other Notes

A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.

Legal Information

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• 3× Dilution and Equilibration Buffer 90 mL

  SDS
• P8340

  Protease Inhibitor Cocktail 1 mL

  SDS

Safety Information

• signalword: Danger
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup
• wgk: WGK 3
• pictograms: GHS05,GHS09
• hcodes: H290,H314,H410,EUH430
• pcodes: P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A