MBD0024

Microbial DNA standard from Thermobifida fusca

Suitable for PCR, sequencing and NGS, 10 ng/μL

• UNSPSC Code: 41105500
• NACRES: NA.51

Properties

• Quality Level: 200
• form: liquid
• concentration: 10 ng/μL
• technique(s): DNA extraction: suitable; DNA sequencing: suitable; PCR: suitable
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Thermobifida fusca genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.T. fusca, is a moderately thermophilic gram-positive soil bacterium, that belongs to actinobacteria, with optimum growth at 55-60°C.2,3 The genome of T. fusca has a high GC content (67.5%) 3 and therefore it may serve as a quality marker for the PCR stage in the NGS workflow. It is a major degrader of plant cell walls and has been used as a model organism for the study of secreted, thermostable cellulases. 3 Thermostable enzymes from T.fusca have been reported to replace thermochemical pre- treatments of cellulosic biomass in the process of decomposition to biofuels.4T. fusca, being a soil bacterium, is not likely to be found in the human microbiome. Therefore, T. fusca genomic DNA standard can be used as an internal standard for the NGS workflow; as a negative control for sample preparation, or as a reference DNA for sequencing.

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.

Suitability

PCR, sequencing, next generation sequencing

Physical form

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable