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M8945
Matrix Metalloproteinase-9 human
recombinant, >90% (SDS-PAGE), buffered aqueous solution
Synonym(s):
Gelantinase-B, Gelatinase, 95 kDa, MMP-9
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| Size/SKU | Availability | Price |
|---|---|---|
10 μg | Check Cart for Availability | CLP 644,000 |
About This Item
Quality Segment
assay
>90% (SDS-PAGE)
form
buffered aqueous solution
mol wt
apparent mol wt ~93 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MMP9(4318)
Biochem/physiol Actions
MMP-9 degrades the collagens that make up the extracellular matrix, which has a role in the control of angiogenesis.
Physical form
Solution in Tris-HCl, pH 7.5, Calcium Chloride, Sodium Chloride, and Brij-35
Analysis Note
The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.
1 of 1
This Item | |||
|---|---|---|---|
| assay >90% (SDS-PAGE) | assay 95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay >90% (SDS-PAGE) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form liquid | form liquid | form lyophilized powder |
| mol wt apparent mol wt ~93 kDa | mol wt calculated mol wt 76 kDa, observed mol wt 92 kDa (The protein migrates as a 92 kDa protein on SDS-PAGE due to glycosylation) | mol wt calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation) | mol wt apparent mol wt ~69 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... MMP9(4318) | Gene Information human ... MMP(4318) | Gene Information human ... MMP(4318) | Gene Information human ... MMP2(4313) |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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What is the Department of Transportation shipping information for this product?
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Is product M8945 (Marix Metalloproteinase-9 Human) endotoxin free?
1 answer-
This product contains < 1.0 EU/ug of the enzyme.
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Does product M8945 (Matrix Metalloproteinase-9 Human) need to be activated?
1 answer-
To activate recombinant human MMP-9, prepare a p-aminophenylmercuric acetate (APMA) solution in DMSO. Add the AMPA solution to the MMP-9 to a final APMA concentration of 1 mM. Incubate at 37°C. for 16 - 24 hours.
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What is the molecular weight of product M8945 (Matrix Metalloproteinase-9 Human)
1 answer-
Human MMP-9 consists of a 688 amino acid residue recombinant protein which has a predicted molecular mass of approximately 77 kDa. By SDS-PAGE the apparent molecular mass is approximately 93 kDa.
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