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M8662

Sigma-Aldrich

Mineral oil

PCR Reagent

Synonym(s):

Mineral oil for PCR

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About This Item

CAS Number:
8042-47-5
EC Number:
232-455-8
MDL number:
MFCD00131611
UNSPSC Code:
12181504
NACRES:
NA.52

grade

PCR Reagent

form

liquid

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Application

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Features and Benefits

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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