M7683

Anti-phospho-MAP Kinase Kinase 1/2 (pSer^217/221) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-phospho-MEK 1/2 (pSer^217/221)
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD02097160
• Conjugate: unconjugated
• Clone: polyclonal
• Application: FACS, ICC, IHC (p), IP, WB
• Citations: 10

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 45 kDa
• species reactivity: human, rat, mouse
• technique(s): flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells., immunocytochemistry: 1:1,000 using HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma, immunoprecipitation (IP): 1:50, western blot: 1:1,000 using serum-treated NIH-3T3 cells
• UniProt accession no.: P28482
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer217/pSer221)
• Gene Information: human ... MAPK1(5594) ; mouse ... Mapk1(26413) ; rat ... Mapk1(116590)

Description

General description

MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells.
The antibody specifically recognizes MEK1/2 (45 kDa) only when phosphorylated at Ser²¹⁷ and Ser²²¹. It reacts strongly with MEK1/2 phosphorylated only at Ser²¹⁷, but less when phosphorylated only at Ser²²¹. It shows not cross reaction with any other related family members including SEK.

Immunogen

synthetic phosphopeptide Ser^217/221 corresponding to residues around Ser^217/221 of human MEK 1/2.

Application

Anti-phospho-MAP Kinase Kinase 1/2 (MEK1/2) has a wide range of applications. The recommended working dilution for immunoblotting in serum treated NIH3T3 cells is 1:1000. For immunoprecipitation, a working dilution of 1:50 may be used. For detection by immunohistochemistry in paraffin-embedded human breast carcinoma sections, antibody dilution of 1:100 may be used. For immunocytochemistry (immunofluorescence), in HeLa cells, a working dilution of 1:1000 may be used.

Biochem/physiol Actions

Detects MEK 1/2 when phosphorylated at Ser²¹⁷ and Ser²²¹. It does not react with non-phosphorylated MEK 1/2, SEK (MKK4), MKK3, or MKK6.

Physical form

Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable