M5409

Mouse IgG2a Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

• NACRES: NA.46
• UNSPSC Code: 12352203
• Clone: UPC-10, monoclonal
• Technique(s): flow cytometry: suitable
• Application: FACS
• Citations: 72

Properties

• biological source: mouse
• Quality Segment: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UPC-10, monoclonal
• form: buffered aqueous solution
• technique(s): flow cytometry: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Application

Mouse IgG2a Isotype Control from murine myeloma has been used in:

• immunoprecipitation
• enzyme-linked immunosorbent assay (ELISA)[1]
• fluorescence microscopy
• indirect immunocytochemistry

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.

When evaluated in flow cytometry, the product did not stain human peripheral blood lymphocytes (PBLs). Anti-Mouse IgG (whole molecule), F(ab′)2 fragment FITC, Catalog Number F2883, along with 1 mg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)