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M2154

Sigma-Aldrich

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.0-7.6 (25 °C, 209 g/L)

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • to culture rumen epithelial cells from yearling Speckle Park beef heifers
  • as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
  • as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

Reconstitution

Supplement with 0.1 g/L L-glutamine.

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supplement

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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E Held-Hoelker et al.
Theriogenology, 96, 145-152 (2017-05-24)
Lipid accumulation is associated with reduced embryonic quality, causing limited survival after cryopreservation. Therefore, in the present study we aimed to reveal the effects of supplementation of a lipid reducing agent, l-carnitine and the removal of fatty acids during in vitro
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Nigel J Brand et al.
Methods in molecular biology (Clifton, N.J.), 633, 113-124 (2010-03-06)
We describe a method of isolating and maintaining primary cultures of mouse neonatal cardiac myocytes (NCM). This is derived from the well-established procedure for making NCM cultures from rat neonates by sequential digestion of rat ventricular myocardial pieces using a
Melisa Gomez-Velazquez et al.
PLoS genetics, 13(8), e1006985-e1006985 (2017-08-29)
Cardiac progenitors are specified early in development and progressively differentiate and mature into fully functional cardiomyocytes. This process is controlled by an extensively studied transcriptional program. However, the regulatory events coordinating the progression of such program from development to maturation
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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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