Recommended Products
General description
Firefly luciferase is one of the most utilized reporter genes for the study of gene expression. It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods. This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg2+. The fluorescent product is quantified to measure its activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Paola Monti et al.
Scientific reports, 10(1), 18427-18427 (2020-10-30)
Chronic lymphocytic leukaemia (CLL) is characterised by a heterogeneous clinical course. Such heterogeneity is associated with a number of markers, including TP53 gene inactivation. While TP53 gene alterations determine resistance to chemotherapy, it is not clear whether they can influence
Jaya Mishra et al.
Journal of the American Society of Nephrology : JASN, 14(10), 2534-2543 (2003-09-30)
Acute renal failure (ARF) secondary to ischemic injury remains a common and potentially devastating problem. A transcriptome-wide interrogation strategy was used to identify renal genes that are induced very early after renal ischemia, whose protein products might serve as novel
Stephen T Smale
Cold Spring Harbor protocols, 2010(5), pdb-pdb (2010-05-05)
When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes
Kai Qian et al.
Biotechnology letters, 37(11), 2229-2235 (2015-07-15)
To improve the bioactivity and increase the N-terminal homogeneity of a glucagon-like peptide-1 (GLP-1) analogue expressed in Pichia pastoris. The GLP-1 analogue. GGH, consisting of two tandem mutant GLP-1 (GLP-1[A2G]) fused with the N-terminus of human serum albumin (HSA), was
Rachel S Edgar et al.
Nature, 485(7399), 459-464 (2012-05-25)
Cellular life emerged ∼3.7 billion years ago. With scant exception, terrestrial organisms have evolved under predictable daily cycles owing to the Earth's rotation. The advantage conferred on organisms that anticipate such environmental cycles has driven the evolution of endogenous circadian rhythms
Articles
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service