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ISO2

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Synonym(s):

Mouse Antibody Isotyping Kit

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1 kit
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CLP 457,000

About This Item

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
Storage temp.:
2-8°C
Shipped in:
wet ice

CLP 457,000


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Quality Segment

usage

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Biochem/physiol Actions

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

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This Item
C7988A7554SAB4200669
description

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

description

clone 7-23, purified from hybridoma cell culture

description

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

description

clone DI-22, purified from hybridoma cell culture

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

indirect ELISA: suitable, immunoprecipitation (IP): suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunocytochemistry: suitable, western blot: 0.5-1 μg/mL using recombinant Cre recombinase

technique(s)

western blot (chemiluminescent): 1:100,000, dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, direct ELISA: 1:25,000

technique(s)

ELISA: 0.15-0.3 μg/mL

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

usage

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

usage

-

usage

-

usage

-


Kit Components Only

Product No.
Description

  • goat antisera to mouse IgA .2 mL

  • goat antisera to mouse IgG3 .2 mL

  • goat antisera to mouse IgG1 .2 mL

  • goat antisera to mouse IgG2a .2 mL

  • goat antisera to mouse IgG2b .2 mL

  • goat antisera to mouse IgM .2 mL

Storage Class

10 - Combustible liquids



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