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GE28-9852-55

Protein G GraviTrap™

Cytiva 28-9852-55, pack of 10 × 1 mL

Synonym(s):

GraviTrap for Protein G, High Performance GraviTrap, Protein G GraviTrap

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

packaging

pack of 10 × 1 mL

manufacturer/tradename

Cytiva 28-9852-55

storage condition

(20% Ethanol)

bed volume

1 mL

capacity

~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

General description

Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose™ 4 Fast Flow.

Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Application

Fast and efficient manual purification of monoclonal and polyclonal antibodies

Features and Benefits

  • Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
  • High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniel Neumeier et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(18), e2113766119-e2113766119 (2022-04-30)
The capacity of humoral B cell-mediated immunity to effectively respond to and protect against pathogenic infections is largely driven by the presence of a diverse repertoire of polyclonal antibodies in the serum, which are produced by plasma cells (PCs). Recent

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocols

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service