G8634

Guanosine 5′-[γ-thio]triphosphate tetralithium salt

≥90% (contains < 10% GDP, HPLC), powder

• Synonym(s): GTPγS tetralithium salt, GTP-γ-S-Li₄, Guanosine 5′-O-(3-thiotriphosphate) tetralithium salt
• Empirical Formula (Hill Notation): C₁₀H₁₂Li₄N₅O₁₃P₃S
• CAS Number: 94825-44-2
• Molecular Weight: 562.98
• Beilstein: 8182249
• EC Number: 305-606-1
• MDL number: MFCD00135151
• UNSPSC Code: 41106305
• PubChem Substance ID: 24895344
• NACRES: NA.77

Properties

• Assay: ≥90% (contains < 10% GDP, HPLC)
• form: powder
• color: white to off-white
• solubility: H₂O: ≥75 mg/mL (Solutions are very unstable; prepare immediately prior to use.)
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Li+].[Li+].[Li+].[Li+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=S)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O13P3S.4Li/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(26-9)1-25-29(19,20)27-30(21,22)28-31(23,24)32;;;;/h2-3,5-6,9,16-17H,1H2,(H,19,20)(H,21,22)(H2,23,24,32)(H3,11,13,14,18);;;;/q;4*+1/p-4/t3-,5-,6-,9-;;;;/m1..../s1
• InChI key: AMQXJFWJOAWCPV-ZVQJTLEUSA-J

Description

Application

Guanosine 5′-[γ-thio]triphosphate tetralithium salt has been used:

• as a component of lysis buffer
• in oocyte and mammalian cell patch-clamp experiments to keep the G proteins in a constantly active state
• to couple with G- protein α, to study its effect on kinase activity

Biochem/physiol Actions

GTP-γ-S is a non-hydrolyzable GTP analog, known for its role as a G-protein activator. It has recently been shown to protect proteins from proteolytic degradation, stimulate GLUT4 translocation in a tyrosine kinase-dependent manner, stimulate phospholipases and induce actin polymerization in vitro.

Features and Benefits

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves