F8512

Anti-Fibrinogen antibody produced in goat

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162408
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), QPA
• Citations: 18

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: human
• technique(s): indirect ELISA: 1:10,000, quantitative precipitin assay: 2.0 mg/mL
• UniProt accession no.: P02671
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FGA(2243), FGB(2244), FGG(2266)

Description

General description

Fibrinogen is a thrombin-coagulable soluble plasma 340 kDa glycoprotein, composed of paired sets of three subunits i.e. α, β, γ. It plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. Among three subunits, β and γ subunits contain one N-glycosylation site, which is occupied by a biantennary N-glycan. It contains three pairs of disulfide-bonded chains called α, β and γ which further folded into four structural domains: the D, E, connector, and the COOH-terminal region of the Aα chain.

The fibrinogen gene cluster consists of fibrinogen α, β and γ chains. It is localized on human chromosome locus 4q31.3−4q32.1.

Immunogen

human fibrinogen

Application

Anti-Fibrinogen antibody is suitable for immunostaining in fibrin deposition analysis of mouse livers and capturing antibodies in the sandwich ELISA. It is also suitable for indirect ELISA at a dilution of 1:10,000 and quantitative precipitin assay at 2.0mg/mL concentration.

Anti-Fibrinogen antibody produced in goat has been used in:

• western blotting detection in human colon adenocarcinoma cell line
• detecting fibrinogen in plasma
• immunoassay of human platelet free plasma (PFP)

Biochem/physiol Actions

The antiserum has been determined to be immunospecific for fibrinogen by immunoelectrophoresis versus human plasma and fibrinogen.

Plasmin attacks the Aα chain COOH domain to produce the heterogeneous fragment X. Multiple round of degradation ended with terminal digestion products−fragments D and E which represent the major globular domains in fibrinogen. Mutations in this gene lead to several disorders, including hypofibrinogenemia and afibrinogenemia.

Preparation Note

treated to remove lipoproteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg