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Fluorescein isothiocyanate isomer I

Name: Fluorescein isothiocyanate isomer I
Brand: SIGMA

≥97.5% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₁NO₅S

CAS Number:

3326-32-7

Molecular Weight:

389.38

NACRES:

NA.47

PubChem Substance ID:

24894860

UNSPSC Code:

12171500

EC Number:

222-042-0

MDL number:

MFCD00005063

Beilstein/REAXYS Number:

1407295

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Properties

Product Name

Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

Quality Segment

100

assay

≥97.5% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to very dark yellow

mp

>360 °C (lit.)

solubility

acetone: soluble 10 mg/mL

fluorescence

λ_ex 492 nm; λ_em 518 nm (green)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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1 of 1

This Item	F7250	34321-M	F3651
Sigma-Aldrich F4274 Fluorescein isothiocyanate isomer I	Sigma-Aldrich F7250 Fluorescein isothiocyanate isomer I	Sigma-Aldrich 34321-M FITC, Isomer I	Sigma-Aldrich F3651 Fluorescein 5(6)-isothiocyanate
technique(s) titration: suitable	technique(s) titration: suitable	technique(s) -	technique(s) titration: suitable
color yellow to very dark yellow	color orange to dark orange	color orange-red	color yellow to orange
fluorescence λ_ex 492 nm; λ_em 518 nm (green)	fluorescence λ_ex 492 nm; λ_em 518 nm (green)	fluorescence -	fluorescence -
solubility acetone: soluble 10 mg/mL	solubility acetone: 1 mg/mL	solubility DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble	solubility acetone: 1 mg/mL, clear, light yellow to yellow
form powder	form powder	form solid	form powder
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) diagnostic assay manufacturing hematology histology

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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