F1180

Anti-Flotillin 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-FLOT1, Anti-Reggie-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01633353
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, WB
• Citations: 27

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~48 kDa
• species reactivity: rat, mouse, human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using human HEK-293K cells, western blot: 0.5-1 μg/mL using whole extract of mouse lung, western blot: 1-2 μg/mL using whole extract of rat lung
• UniProt accession no.: O75955
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FLOT1(10211) ; mouse ... Flot1(14251) ; rat ... Flot1(64665)

Description

General description

Flotillin 1 encodes a caveolae-associated, integral membrane protein. Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction.

Flotillin 1(FLOT1), also known as reggie-2, is an estrogen responsive gene mapped to human chromosome 6p21.33. This gene codes for an integral membrane protein and a component of lipid rafts.

Flotillins are lipid raft associated proteins expressed mainly in adipose tissue, striated muscle tissues, and lung. It has a crucial role in several cellular processes like phagocytosis, endocytosis, insulin signalling and neuronal regeneration. Anti-flotillin 1 antibody can be used in immunobloting and immunolocalization. Rabbit anti- flotillin 1 antibody reacts specifically with flotillin 1 of rat, mouse and human.

Immunogen

synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.

Application

Anti-flotillin 1 antibody can be used in immunofluorescence and western blotting.

Anti-flotillin 1 antibody produced in rabbit has been used in immunoblot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Flotillin 1 (FLOT1) has a role in endocytosis. It also functions in growth factor signaling and activates mitogen-activated protein kinase signaling. In addition, it is also involved in phagocytosis, regulation of actin cytoskeleton and membrane trafficking. The protein has been associated with tumor progression and is expressed in many cancers, including breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)