C4982

Cytochrome P450 human

3A4 isozyme microsomes, with P450 reductase and cytochrome b₅, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• Synonym(s): Cytochrome P450 enzyme, Human cytochrome P450, P450 enzyme
• UNSPSC Code: 12161501
• NACRES: NA.47
• EC Number: 1.6.2.4 (BRENDA, IUBMB)
• MDL number: MFCD03456520
• Biological source: human
• Recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• Concentration: ≥2 mg/mL (Bichinchonic acid method)

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• form: solution
• mol wt: 45-60 kDa
• packaging: vial of ~0.5 nmol, vial of ≥25 units
• concentration: ≥2 mg/mL (Bichinchonic acid method)
• technique(s): co-immunoprecipitation (co-IP): suitable
• solubility: water: soluble
• suitability: suitable for molecular biology
• UniProt accession no.: P08684
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP3A4(1576)

Description

General description

Research area: IMMUNO AND CKS

Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand.

Application

Cytochrome P450 human has been used in in vitro binding assays to understand protein dimerization via haem–haem stacking and its significance in cancer.[1] It has also been used to study in vitro binding between PGRMC1 and CYP3A4.[2]

Biochem/physiol Actions

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.

Cytochrome P450 (CYP) enzymes are associated with many reactions including O-dealkylation, epoxidation, S-oxidation, and hydroxylation.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Other Notes

Microsomes containing recombinant human CYP3A4 and recombinant rabbit NADPH-P450 reductase

One unit will convert 1 nanomole of testosterone to 6β-hydroxytestosterone per minute at pH 7.4 at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable