C3649

CHAPSO

≥98%

• Synonym(s): 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate
• Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₈S
• CAS Number: 82473-24-3
• Molecular Weight: 630.88
• Beilstein: 5842642
• MDL number: MFCD00081079
• UNSPSC Code: 12161900
• PubChem Substance ID: 24892634
• NACRES: NA.25

Properties

• description: zwitterionic
• Assay: ≥98%
• form: powder
• mol wt: micellar avg mol wt 7000
• aggregation number: 11
• technique(s): protein quantification: suitable
• conductivity: ≤100 μmho per cm at 24 °C (0.5 M aqueous solution)
• CMC: 8 mM (20-25°C)
• mp: 184-186 °C (lit.)
• transition temp: cloud point 90 °C
• SMILES string: [H][C@@]12C[C@H](O)CC[C@]1(C)[C@@]3([H])C[C@H](O)[C@]4(C)[C@]([H])(CC[C@@]4([H])[C@]3([H])[C@H](O)C2)[C@H](C)CCC(=O)NCCC[N+](C)(C)CC(O)CS([O-])(=O)=O
• InChI: 1S/C32H58N2O8S/c1-20(7-10-29(39)33-13-6-14-34(4,5)18-23(36)19-43(40,41)42)24-8-9-25-30-26(17-28(38)32(24,25)3)31(2)12-11-22(35)15-21(31)16-27(30)37/h20-28,30,35-38H,6-19H2,1-5H3,(H-,33,39,40,41,42)/t20-,21+,22-,23?,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: GUQQBLRVXOUDTN-XOHPMCGNSA-N

Description

General description

CHAPSO is a non-denaturing zwitterionic bile salt derivative, derived from Chaps by the addition of a functional hydroxyl group.

Application

A nondenaturing zwitterionic detergent with characteristics similar to CHAPS, although it is more soluble due to a more polar head group. Useful for the solubilization of integral membrane proteins.

CHAPSO has been used:

• in a study to assess the processes of liposome solubilization and reconstitution of two transport proteins
• in a study to investigate a novel bicelle system for orienting macromolecules over a wide temperature range
• as a mixture with dilauroyl phosphatidylcholine (DLPC) in solid-state nuclear magnetic resonance (NMR) technique to study membrane-associated proteins

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves