C3640

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

• Synonym(s): Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
• CAS Number: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-576-6
• MDL number: MFCD00081467
• EC Number: 4.2.1.1 (BRENDA, IUBMB)
• Specific activity: ≥2,000 W-A units/mg protein

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥2,000 W-A units/mg protein
• pI: ~5.4
• storage temp.: −20°C

Description

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme. The enzyme has also been used to generate CD₄⁺ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in pathologies such as glaucoma, epilepsy and Parkinson′s disease.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)