C1999
CMP-Sialic Acid Synthetase from Neisseria meningitidis group B
recombinant, expressed in E. coli BL21, ≥10 units/mg protein
Synonym(s):
CTP: N-Acylneuraminate cytidylyltransferase
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| Size/SKU | Availability | Price |
|---|---|---|
10 units | Check Cart for Availability | CLP 275,000 |
About This Item
Specific activity:
≥10 units/mg protein
Recombinant:
expressed in E. coli BL21
CLP 275,000
Check Cart for Availability
recombinant
expressed in E. coli BL21
Quality Segment
form
lyophilized solid
specific activity
≥10 units/mg protein
mol wt
26.0 kDa
shipped in
dry ice
storage temp.
−20°C
General description
Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.
Application
The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.[1]
Biochem/physiol Actions
Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase.
Physical form
Supplied as a lyophilized powder containing Tris-HCl and NaCl.
Analysis Note
Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).
Other Notes
One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥10 units/mg protein | specific activity ≥2 units/mg protein | specific activity ≥5 units/mg protein | specific activity ≥3.0 units/mg protein |
| recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 |
| form lyophilized solid | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| mol wt 26.0 kDa | mol wt 46.4 kDa | mol wt 56.8 kDa | mol wt 33.4 kDa |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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