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C0230

Sigma-Aldrich

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse

~1 mg/mL, clone 28F3, purified immunoglobulin, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD01864149
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

28F3, monoclonal

form

buffered aqueous glycerol solution

mol wt

antigen 28-30 kDa

species reactivity

bovine, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1:5,000 using normal human fibroblasts or MDBK cells

isotype

IgG1

UniProt accession no.

Q96L46

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CAPNS1(826) , CAPNS2(84290)

General description

Calpain small subunit 1 (Capns1) is a small regulatory subunit associated with the μ-calpain (calpain 1) and m-calpain (calpain 2). The CAPNS1 gene is mapped to human chromosome 19q13.12.

Immunogen

purified calpain from human placenta.

Application

Monoclonal Anti-m/μ Calpain, Small Subunit antibody produced in mouse has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Calpain small subunit 1 (Capns1) participates in the actin cytoskeleton rearrangement and cell migration events. It is crucial for embryonic development. Capns1 interacts with the Ras GTPase activating protein Src homology 3 domain (SH3) (RasGAP-SH3) domain to regulate cell migration and cell survival. It is essential for calpain activity. Capns1 is associated with the oncogenesis of many tumor types including hepatocellular, colorectal, ovarian, and breast cancer.

Physical form

Solution in 0.02 M sodium phosphate, pH 7.5, and 0.15 M sodium chloride containing 50% glycerol and 3 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria E Saez et al.
Drug discovery today, 11(19-20), 917-923 (2006-09-26)
The calpain family is a group of cysteine proteases unique in their dependency on calcium to attain functionally active forms. Calpains are involved in a wide range of cellular calcium-regulated functions, including signal transduction, cell proliferation and differentiation, and apoptosis.
Chuanwen Zhao et al.
Experimental cell research, 395(2), 112220-112220 (2020-08-11)
Capn4, a small regulatory subunit of the calpain proteolytic system, functions as a potential tumor promoter in several cancers. However, the biological functions and molecular mechanisms of Capn4 in gastric cancer (GC) remain poorly understood. In the current study, we
Perayot Pamonsinlapatham et al.
Cellular signalling, 20(11), 2119-2126 (2008-09-02)
Ras GTPase-activating protein (RasGAP) is hypothesized to be an effector of oncogenic Ras stimulating numerous downstream cellular signaling cascades involved in survival, proliferation and motility. In this study, we identified calpain small subunit-1 (Capns1) as a new RasGAP-SH3 domain binding
Elena Marcassa et al.
Biology open, 6(5), 551-562 (2017-03-18)
CAPNS1 is essential for stability and function of the ubiquitous calcium-dependent proteases micro- and milli-calpain. Upon inhibition of the endoplasmic reticulum Ca

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