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B7632

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

chromogenic, protease substrate, ≥98% (TLC), powder

Synonym(s):

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride

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5 mg
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CLP 193,000
10 mg
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CLP 333,000
50 mg
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CLP 1,160,000

About This Item

Empirical Formula (Hill Notation):
C33H40N8O6 · HCl
CAS Number:
54799-93-8
Molecular Weight:
681.18
NACRES:
NA.32
PubChem Substance ID:
24892011
UNSPSC Code:
12352204
MDL number:
MFCD00038397
Beilstein/REAXYS Number:
3027332

CLP 193,000

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Product Name

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

General description

A substrate for trypsin, thrombin and reptilase.
N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.[1]

Application

  • N

  • -Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae[2]
  • for the thrombin, recombinant and native batroxobin from snake venom[3]
  • for fibrinolytic enzyme aprE2 in amidolytic activity assay[4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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1 of 1

This Item
B3279B2133B4875
N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride protease substrate

Sigma-Aldrich

B7632

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride ≥98% (HPLC)

Sigma-Aldrich

B3279

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride protease substrate

Sigma-Aldrich

B2133

N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride ≥98%

Sigma-Aldrich

B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

assay

≥98%

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

solubility

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

solubility

methanol: 25 mg/mL

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5772
BCCD7744
BCCB9524
BCBV9946
BCBD4150V

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Protocols

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

S Rosén
Hamostaseologie, 25(3), 259-266 (2005-08-23)
Chromogenic peptide substrates were developed more than 30 years ago. Although the use of chromogenic substrate methods in coagulation and fibrinolysis diagnostics was not as rapidly implemented as initially believed, they are now well established for several analytes such as
[Clinical evaluation of prothrombin assay using chromogenic peptide substrates (author's transl)].
Y Akiyama et al.
Rinsho byori. The Japanese journal of clinical pathology, 28(11), 1131-1135 (1980-11-01)
Milton Erthal et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 152(1), 54-59 (2008-10-14)
The production of enzymes and the colonization of leaves by Leucoagaricus gongylophorus were investigated to further understand the digestive interactions of leaf-cutting ant colonies. The enzymes detected were indicative of a saprophytic origin of this fungus, producing all the enzymes
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Global Trade Item Number

SKUGTIN
B7632-50MG04061832912806
B7632-5MG04061833442012
B7632-10MG04061833442005

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