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A7089

Sigma-Aldrich

Accumax™ solution

sterile-filtered, suitable for cell culture

Synonym(s):

Cell detachment solution

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100 ML
CLP 180,000
500 ML
CLP 646,000

CLP 180,000

Estimated to ship onApril 14, 2025

Request a Bulk Order
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100 ML
CLP 180,000
500 ML
CLP 646,000

About This Item

MDL number:
MFCD01632904
UNSPSC Code:
12352204
NACRES:
NA.75

CLP 180,000

Estimated to ship onApril 14, 2025

Request a Bulk Order

sterility

sterile-filtered

Quality Level

200

form

solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

pH

6.8-7.8

shipped in

dry ice

storage temp.

−20°C

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
TM=["Accumax"] solution has been used:
  • for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells[1]
  • for dissociation of retinal pigmented epithelial cells prior to passaging[2]
  • for dissociation of islets from pancreas[3]

Biochem/physiol Actions

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Features and Benefits

Ready-to-use sterile liquid for in vitro cell applications

Caution

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.

Preparation Note

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000407379
0000407379
0000406897
0000369798
0000406897

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Benjamin T Dodsworth et al.
Scientific reports, 10(1), 10542-10542 (2020-07-01)
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells
Kaiser JP, et al.
Journal of King Saud University - Science, 25(1), 15-27 (2013)
Natalja Funk et al.
Scientific reports, 9(1), 4515-4515 (2019-03-16)
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role of LRRK2 mutations remain uncertain. Here, we investigated the role of LRRK2 in intracellular signal
Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
Geng Z, et al.
PLoS ONE, 12(3), e0173575-e0173575 (2017)
Chronic beta-cell depolarization impairs beta-cell identity by disrupting a network of ca2+-regulated genes
Stancill JS, et al.
Diabetes, 66(8), 2175-2187 (2017)
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Questions

1–4 of 4 Questions  

  1. Is your accumax gmp grade and made in a gmp compliant facility and is animal free?

    1 answer

    1. This Accumax™ solution does not contain mammalian- or bacterial-derived products. It is not a GMP grade. This product is assigned a quality level of 200. Products with quality level of 200 are not suitable for GMP. Products with quality levels 400 or higher are suitable for GMP.
      Currently, there is no GMP grade product available.

      Please see the quality brochure below for more information:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/193/133/m-clarity-brochure-br3925en-ms.pdf

      Helpful?

  2. What is the size of the bottle in size specifications (inches, centimeters)?

    1 answer

    1. See below for the 100 mL and 500 mL bottle dimensions:
      100 mL
      Height: 104.1 mm
      Height w/cap: 108.8 mm
      Opening inner diameter: 28.4 mm
      Outer thread diameter: 37.3 mm

      500 mL
      Height: 173.2 mm
      Height w/cap: 177.8 mm
      Opening inner diameter: 28.4 mm
      Outer thread diameter: 38 mm

      Helpful?

  3. What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?

    1 answer

    1. Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells.  The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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