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A5043

Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Synonym(s):

ADA, adenosine aminohydrolase, Adenosine aminohydrolase

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About This Item

CAS Number:
9026-93-1
Enzyme Commission number:
3.5.4.4 (BRENDA, IUBMB)
MDL number:
MFCD00081287
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine spleen

Quality Level

200

type

Type X

form

buffered aqueous glycerol solution

specific activity

≥130 units/mg protein

mol wt

32.5-33 kDa

cation traces

NH4+: ≤10 μg/mg protein

UniProt accession no.

P56658

foreign activity

alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Gene Information

cow ... ADA(280712)

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General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adenosine reagent-free detection by co-immobilization of adenosine deaminase and phenol red on an optical biostrip
Bartzoka F, et al.
Biotechnology Journal, 10(1), 136-142 (2015)
Ying Cheng et al.
Diabetes, 59(1), 17-25 (2009-10-17)
White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet
Yeon-Mi Lee et al.
Biophysical chemistry, 172, 18-25 (2013-01-22)
Human ADAR1, which has two left-handed Z-DNA binding domains, preferentially binds Z-DNA rather than B-DNA with a high binding affinity. Z-DNA can be induced in long genomic DNA by Z-DNA binding proteins through the formation of two B-Z junctions with
George R Thompson et al.
Chest, 143(3), 776-781 (2012-11-29)
In a patient with positive serum serology for coccidioidomycosis, the differential diagnosis of concurrent pleural effusions can be challenging. We, therefore, sought to clarify the performance characteristics of biochemical, serologic, and nucleic-acid-based testing in an attempt to avoid invasive procedures.
Neutropenia and myeloid dysplasia in a patient with delayed-onset adenosine deaminase deficiency.
Keiko Nomura et al.
Pediatric blood & cancer, 60(5), 885-886 (2013-01-22)
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