50185

Anti-Mouse-IgG - Atto 647N antibody produced in goat

contains 50% glycerol as stabilizer

• Synonym(s): Atto 647N-Anti-Mouse-IgG antibody produced in goat
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD06798561
• Conjugate: Atto 647N conjugate
• Clone: polyclonal
• Application: IF
• Citations: 50

Properties

• conjugate: Atto 647N conjugate
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 50% glycerol as stabilizer
• species reactivity: mouse
• concentration: ≥0.8 mg/mL IgG
• technique(s): immunofluorescence: 5 μg/mL
• fluorescence: λ_ex 647 nm; λ_em 665 nm in PBS
• suitability: in accordance for fluorescence
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Atto 647N-goat anti-mouse IgG associates with mouse IgGs.

Immunogen

mouse IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.Atto 647N goat anti-rabbit IgG was used as the secondary antibody for immunofluorescene at a concentration of 5μg/ml on cells fixed in 2% formaldehyde.

Physical form

Atto 647 goat anti-mouse IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves