The long-term stability of this product in solution has not been determined. However, a similar Collagenase type IV product, C5138, notes that solutions are stable for several months when stored in aliquots at -20°C. Please see the link below to review the datasheet of a similar item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/876/c5138pis.pdf
C4-BIOC
Collagenase Type IV, Cls IV
Synonym(s):
Collagen A, Type IV Gelatinase
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100 MG
CLP 45,300
1 G
CLP 280,000
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100 MG
CLP 45,300
1 G
CLP 280,000
About This Item
UNSPSC Code:
12352204
NACRES:
NA.78
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General description
Collagenase Type IV, Cls IV is a member of the mammalian extracellular neutral metalloproteinases family. It digests type IV collagen, which is a major structural constituent of the basement membrane.[1]
This product is identical to Biochrom GmbH part numbers C4-22 and C4-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities. Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.
Application
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients[2]
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats[3]
- in the isolation of rat glomerular endothelial cells (GEndCs)[4]
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Yulia Gnainsky et al.
Fertility and sterility, 94(6), 2030-2036 (2010-03-27)
To study whether an injury-induced inflammation might be the mechanism underlying the favorable effect of endometrial biopsy on the implantation rate in in vitro fertilization (IVF) patients. Controlled clinical study. A medical center IVF unit and a research institute. Women
Sina M Schalbetter et al.
Science advances, 8(9), eabi6672-eabi6672 (2022-03-03)
The prefrontal cortex (PFC) is a cortical brain region that regulates various cognitive functions. One distinctive feature of the PFC is its protracted adolescent maturation, which is necessary for acquiring mature cognitive abilities in adulthood. Here, we show that microglia
Liquan Jin et al.
Molecular medicine reports, 27(3) (2023-02-18)
The present study aimed to identify the function and expression of trimethylated protein histone H3 lysine 36 (H3K36)me3 and the upstream specific enzyme histone methyltransferase SET domain containing 2 (SETD2), during the differentiation of hepatic oval cells (HOCs) into cholangiocytes
P Huhtala et al.
The Journal of biological chemistry, 265(19), 11077-11082 (1990-07-05)
The structure of the gene for human 70-kDa type IV collagenase (gelatinase) was determined. Three overlapping genomic clones were isolated and shown to contain 0.4 kilobase (kb) of the 5'-flanking region, the 27-kb structural gene, and 4.5 kb of the
Mingtai Gui et al.
Experimental and therapeutic medicine, 22(6), 1446-1446 (2021-11-02)
Continuous and irreversible cardiac hypertrophy can induce cardiac maladaptation and cardiac remodeling, resulting in increased risk of developing cardiovascular diseases. The present study was conducted to investigate the therapeutic effect of Huoxue Qianyang Qutan recipe (HQQR) on angiotensin II (Ang
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What is the storage condition and shelf life after reconstituting the product?
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How does one solubilize collagenase, and what is the usage concentration for digestion and cell isolation?
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The substance is soluble at 50mg/ml and can be dissolved in water, although it is recommended to use saline, NaCl, DPBS, Hanks, or a culture medium. It is worth noting that in peer-reviewed papers at sial.com, glomerular endothelial cells were isolated at 0.1% in DMEM.
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