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75917

Millipore

Brain Heart Infusion, Porcine

suitable for microbiology

Synonym(s):

BHI Broth, Brain Heart Broth

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

biological source

Porcine heart

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

amino-nitrogen, ≥4.5%
total nitrogen (N), ≥11%

packaging

pkg of 500 g

storage condition

(Tightly closed. Dry.)

ign. residue

≤17%

loss

≤6.0% loss on drying

color

light yellow

pH

6.5-7.5 (25 °C, 2% in H2O)

solubility

H2O: 2%, clear, light yellow (and light brown-yellow and light brown)

application(s)

food and beverages
microbiology

General description

Brain Heart Infusion (BHI), Porcine, is a culture medium commonly used in microbiology for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures.BHI is typically prepared using porcine (pig) brain and heart tissues as the source of the raw materials. The enzymatic digestion of porcine brain-heart infusion provides amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.

Application

Brain Heart Infusion, porcine, is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium for the growth of Streptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes, and Clostridium difficile.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM0779
BCCF0960
BCCD4006
BCBR9165V
BCBQ9344V

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Articles

Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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