LMDH-RO

L-Malate Dehydrogenase (L-MDH)

from pig heart (mitochondrial)

• UNSPSC Code: 12352202
• EC Number: 1.1.1.37 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• Biological source: Porcine heart (mitochondrial)

Properties

• biological source: Porcine heart (mitochondrial)
• form: solution, suspension
• specific activity: ~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
• packaging: pkg of 5 mL (10127914001suspension)
• manufacturer/tradename: Roche
• technique(s): activity assay: suitable
• color: white
• optimum pH: 7.4-7.5(reduction of oxaloacetate), 9.2-9.5(oxidation of malate)
• solubility: water: miscible
• suitability: suitable for Western blot
• NCBI accession no.: NM_001244153.1
• UniProt accession no.: P00346
• application(s): life science and biopharma
• foreign activity: Fumarase <0.01%, GIDH 0.001%, GOT 0.001%, GPT 0.00185%, LDH <0.01%, NADH oxidase <0.001%
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Porcine ... MDH2(397039)

Description

General description

L-malate:NAD⁺ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

Application

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.

Biochem/physiol Actions

The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.

Physical form

Solution in 50% glycerol (v/v), pH approximately 7

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: – phosphate
– arsenate
– Mg²⁺
– Zn²⁺

Analysis Note

Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available